Excellent Sensitivity, Specificity & Reproducibility

ELISA (Enzyme-Linked Immunosorbent Assay) is a technique to detect the presence of antigens in biological samples. An ELISA, like other types of immunoassays, relies on antibodies to detect a target antigen using highly specific antibody-antigen interactions.

Basic ELISA principles: In an ELISA assay, the antigen is immobilized to a solid surface. This is done either directly or via the use of a capture antibody itself immobilized on the surface. The antigen is then complexed to a detection antibody conjugated with a molecule amenable for detection such as an enzyme or a fluorophore.

An ELISA assay is typically performed in a multi-well plate (96- or 384-wells), which provides the solid surface to immobilize the antigen. Immobilization of the analytes facilitates the separation of the antigen from the rest of the components in the sample. This characteristic makes ELISA one of the easiest assays to perform on multiple samples simultaneously.

Features

  • High sensitivity and specificity: It is common for ELISAs to detect antigens at the picogram level in a very specific manner due to the use of antibodies.
  • High throughput: Commercial ELISA kits are normally available in a 96-well plate format. But the assay can be easily adapted to 384-well plates.
  • Easy to perform: Protocols are easy to follow and involve little hands-on time.
  • Quantitative: It can determine the concentration of antigen in a sample.
  • Possibility to test various sample types: serum, plasma, cellular and tissue extracts, urine, and saliva among others.

Types of ELISA Immunoassays

There are four main types of ELISA: direct ELISA, indirect ELISA, sandwich ELISA and competitive ELISA. Each has unique advantages, disadvantages and suitability.


In a direct ELISA, the antigen is immobilized to the surface of the multi-well plate and detected with an antibody specific for the antigen The antibody is directly conjugated to HRP or other detection molecules.

Advantages: ● Short protocol: saves time and reagents. ● No cross-reactivity from secondary antibody.

Disadvantages: ● Potential high background: all proteins in the sample bind to the surface. ● No signal amplification. ● Low flexibility: the primary antibody must be conjugated.

Indirect ELISA is a technique that uses a two-step process for detection, whereby a primary antibody specific for the antigen binds to the target, and a labeled secondary antibody against the host species of the primary antibody binds to the primary antibody for detection. As for direct ELISA assays, the antigen is immobilized to the surface of the multi-well plate. 

The method can also be used to detect specific antibodies in a serum sample by substituting the serum for the primary antibody.

Advantages: ● Signal amplification: several secondary antibodies will bind to the primary antibody. ● High flexibility: the same secondary antibody may be used for several primary antibodies.

Disadvantages: ● Long protocol if compared to direct ELISA. ● Potential cross-reactivity from secondary antibody.

Sandwich ELISA (or sandwich immunoassay) is the most commonly used format. This format requires two antibodies specific for different epitopes of the antigen. These two antibodies are normally referred to as matched antibody pairs. One of the antibodies is coated on the surface of the multi-well plate and used as a capture antibody to facilitate the immobilization of the antigen.  The other antibody is conjugated and facilitates the detection of the antigen.

Advantages: ● High specificity: involves two antibodies detecting different epitopes on the same antigen. ● Suitable for complex samples. ● High flexibility and sensitivity: both direct and indirect methods can be used.

Disadvantages: ● Demanding design: finding two antibodies against the same target that recognize different epitopes and work well together can be challenging at times.

Also known as inhibition ELISA or competitive immunoassay, competitive ELISA assays measure the concentration of an antigen by detection of signal interference. Each of the previous formats can be adapted to the competitive format. 

The sample antigen competes with a reference antigen for binding to a specific amount of labeled antibody. The reference antigen is pre-coated on a multi-well plate and sample is pre-incubated with labeled antibody and added to the wells. Depending on the amount of antigen in the sample, more or less free antibodies will be available to bind the reference antigen. This means the more antigen there is in the sample, the less reference antigen will be detected and the weaker the signal.

Some competitive ELISA kits use labeled antigen instead of a labeled antibody. The labeled antigen and the sample antigen (unlabeled) compete for binding to the primary antibody. The lower the amount of antigen in the sample, the stronger the signal due to more labeled antigen in the well.

Advantages: ● Depends on base ELISA selected. ● Suitable for small antigens.

Disadvantages: ● Depends on base ELISA selected

 
 

BIOS ELISA Immunoassay

BIOS range of ELISAs are well researched, time-tested products with over 30 parameters classified as hormones, steroids, thyroids, tumor markers, diabetes marker, cardiac marker, allergy and anemia, infectious diseases, autoimmune diseases, TORCH and Vitamin D.

For all products :- Storage: 2-8°C | Stability: 12 months.

Product Catalog (Finished Kits)

For OEM/ Bulk packs click HERE

PRODUCT NAME & DETAILS
CAT. NO.
PACK SIZE
PRODUCT NAME
CAT. NO.
PACK SIZE
IFU
MSDS
IFU -->
Hormone
Follicle Stimulating Hormone (FSH) Enzyme Immunoassay Test Kit
IFU MSDS
1401010096 96 Tests
Prolactin (PRL) Enzyme Immunoassay Test Kit
IFU MSDS
1401020096 96 Tests
Luteinizing Hormone (LH) Enzyme Immunoassay Test Kit
IFU MSDS
1401030096 96 Tests
Beta - Human Chorionic Gonadotropin (β-hCG) Enzyme Immunoassay Test Kit
IFU MSDS
1401040096 96 Tests
Anti-Mullerian Hormone Enzyme Immunoassay Test Kit
IFU MSDS
1401050096 96 Tests
 
Steroid
Progesterone Enzyme Immunoassay Test Kit
IFU MSDS
1402010096 96 Tests
Testosterone Enzyme Immunoassay Test Kit
IFU MSDS
1402020096 96 Tests
 
Thyroid
Total Triiodothyronine (T3) Enzyme Immunoassay Test Kit
IFU MSDS
1403010096 96 Tests
Total Thyroxine (T4) Enzyme Immunoassay Test Kit
IFU MSDS
1403020096 96 Tests
Free Triiodothyronine (FT3) Enzyme Immunoassay Test Kit
IFU MSDS
1403030096 96 Tests
Free Thyroxine (FT4) Enzyme Immunoassay Test Kit
IFU MSDS
1403040096 96 Tests
Thyroid Stimulating Hormone (TSH) Enzyme Immunoassay Test Kit
IFU MSDS
1403050096 96 Tests
 
 
Diabetes Marker
Insulin Enzyme Immunoassay Test Kit
IFU MSDS
1405010096 96 Tests
C-Peptide Enzyme Immunoassay Test Kit
IFU MSDS
1405020096 96 Tests
 
Allergy and Anemia
Ferritin Enzyme Immunoassay Test Kit
IFU MSDS
1407010096 96 Tests
Immunoglobulin E (IgE) Enzyme Immunoassay Kit
IFU MSDS
1407020096 96 Tests
 
TORCH
Herpes Simplex Virus 1,2 IgG (HSV 1,2 IgG) Enzyme Immunoassay Kit
IFU MSDS
1408010096 96 Tests
Herpes Simplex Virus 1,2 IgM (HSV 1,2 IgM) Enzyme Immunoassay Kit
IFU MSDS
1408020096 96 Tests
Rubella IgG Enzyme Immunoassay Kit
IFU MSDS
1408090096 96 Tests
Rubella IgM Enzyme Immunoassay Kit
IFU MSDS
1408100096 96 Tests
Toxoplasma IgG (Toxo IgG) Enzyme Immunoassay Test Kit
IFU MSDS
1408110096 96 Tests
Toxoplasma IgM (Toxo IgM) Enzyme Immunoassay Test Kit
IFU MSDS
1408120096 96 Tests
Cytomegalovirus IgG (CMV IgG) Enzyme Immunoassay Test Kit
IFU MSDS
1408140096 96 Tests
Cytomegalovirus IgM (CMV IgM) Enzyme Immunoassay Test Kit
IFU MSDS
1408150096 96 Tests
TORC Avidity Buffer
IFU
1402010096 96 Tests
 
Autoimmune Disease
DsDNA Antibodies (dsDNA) Enzyme Immunoassay Test Kit
IFU MSDS
1409070096 96 Tests
Anti-Cyclic Citrullinated Peptides (anti-CCP) Enzyme Immunoassay Test Kit
IFU MSDS
1409080096 96 Tests
 
Others
25-OH Vitamin D Enzyme Immunoassay Test Kit
1410010096 96 Tests
Hormone
Follicle Stimulating Hormone (FSH) Enzyme Immunoassay Test Kit 1401010096 96 Tests
Prolactin (PRL) Enzyme Immunoassay Test Kit 1401020096 96 Tests
Luteinizing Hormone (LH) Enzyme Immunoassay Test Kit 1401030096 96 Tests
Beta - Human Chorionic Gonadotropin (β-hCG) Enzyme Immunoassay Test Kit 1401040096 96 Tests
Anti-Mullerian Hormone Enzyme Immunoassay Test Kit 1401050096 96 Tests
 
Steroid
Progesterone Enzyme Immunoassay Test Kit 1402010096 96 Tests
Testosterone Enzyme Immunoassay Test Kit 1402020096 96 Tests
 
Thyroid
Total Triiodothyronine (T3) Enzyme Immunoassay Test Kit 1403010096 96 Tests
Total Thyroxine (T4) Enzyme Immunoassay Test Kit 1403020096 96 Tests
Free Triiodothyronine (FT3) Enzyme Immunoassay Test Kit 1403030096 96 Tests
Free Thyroxine (FT4) Enzyme Immunoassay Test Kit 1403040096 96 Tests
Thyroid Stimulating Hormone (TSH) Enzyme Immunoassay Test Kit 1403050096 96 Tests
 
Diabetes Marker
Insulin Enzyme Immunoassay Test Kit 1405010096 96 Tests
C-Peptide Enzyme Immunoassay Test Kit 1405020096 96 Tests
 
Allergy and Anemia
Ferritin Enzyme Immunoassay Test Kit 1407010096 96 Tests
Immunoglobulin E (IgE) Enzyme Immunoassay Kit 1407020096 96 Tests
 
TORCH
Herpes Simplex Virus 1,2 IgG (HSV 1,2 IgG) Enzyme Immunoassay Kit 1408010096 96 Tests
Herpes Simplex Virus 1,2 IgM (HSV 1,2 IgM) Enzyme Immunoassay Kit 1408020096 96 Tests
Rubella IgG Enzyme Immunoassay Kit 1408090096 96 Tests
Rubella IgM Enzyme Immunoassay Kit 1408100096 96 Tests
Toxoplasma IgG (Toxo IgG) Enzyme Immunoassay Test Kit 1408110096 96 Tests
Toxoplasma IgM (Toxo IgM) Enzyme Immunoassay Test Kit 1408120096 96 Tests
Cytomegalovirus IgG (CMV IgG) Enzyme Immunoassay Test Kit 1408140096 96 Tests
Cytomegalovirus IgM (CMV IgM) Enzyme Immunoassay Test Kit 1408150096 96 Tests
TORC Avidity Buffer 1402010096 96 Tests
 
Autoimmune Disease
DsDNA Antibodies (dsDNA) Enzyme Immunoassay Test Kit 1409070096 96 Tests
Anti-Cyclic Citrullinated Peptides (anti-CCP) Enzyme Immunoassay Test Kit 1409080096 96 Tests
 
Others
25-OH Vitamin D Enzyme Immunoassay Test Kit 1410010096 96 Tests